报道了一种利用单一波长激发的同时产生光声和荧光信号的显微成像系统, 本成像系统具有超高的成像分辨率(<6 μm)。借助外源的造影剂在近红外的吸收特性, 利用光声-荧光显微成像系统对活体肿瘤进行光声/荧光成像。实验结果表明, 光声-荧光显微镜在早期肿瘤的成像和检测等方面具有潜在的应用价值。因此, 通过研究和选择适当的双模态造影剂, 该系统在不同病理模型中可以提供更准确的组织信息及生理参数。

Photoacoustic therapy, using the photoacoustic effect of agents for selectively killing tumor cells, has shown promising for treating tumor. Utilization of high optical absorption probes can help to effectively improve the photoacoustic therapy efficiency. Herein, we report a novel highabsorption photoacoustic probe that is composed of indocyanine green (ICG) and graphene oxide (GO), entitled GO-ICG, for photoacoustic therapy. The attached ICG with narrow absorption spectral profile has strong optical absorption in the infrared region. The absorption spectrum of the GO-ICG solution reveals that the GO-ICG particles exhibited a 10-fold higher absorbance at 780nm (its peak absorbance) as compared with GO. Importantly, ICG's fluorescence is quenched by GO via fluorescence resonance energy transfer. As a result, GO-ICG can high-efficiently convert the absorbed light energy to acoustic wave under pulsed laser irradiation. We further demonstrate that GO-ICG can produce stronger photoacoustic wave than the GO and ICG alone. Moreover, we conjugate this contrast agent with integrin α_vβ₃ mono-clonal antibody to molecularly target the U87-MG human glioblastoma cells for selective tumor cell killing. Finally, our results testify that the photoacoustic therapy efficiency of GO-ICG is higher than the existing photoacoustic therapy agent. Our work demonstrates that GO-ICG is a high-efficiency photoacoustic therapy agent. This novel photoacoustic probe is likely to be an available candidate for tumor therapy.